We investigated cold sensitivity improvement effects of acupuncture in young women based on changes in skin surface temperature and pulse wave amplitude over time after cold-water loading. We measured these parameters on subjects’ hands after cold-water loading. The subjects were divided between a self-care group that underwent acupuncture and a control group that did not. Thereafter, we compared the measured temperature and amplitude between the two groups. The skin surface temperatures were higher in the self-care group 2 min after cold-water loading. The pulse wave amplitudes were higher in the self-care group 1 and 2 min after the first round, and 6, 8, and 10 min after the second round of cold-water loading. The cold-water loading tests revealed stabilization of autonomic nervous function in the self-care group.