This study aimed to investigate the kinematic profiles of the lumbar spine and pelvis, and muscular activities of the lower back and abdominal muscles while walking on high-heeled shoes in young women. Eight female college students were asked to walk on a flat floor at free walking speed while wearing heels of four different heights (0, 3, 6, and 9 cm). Sagittal angular displacements of the lumbar spine and pelvis and the surface electromyographic (EMG) activities of the lower trunk muscles were recorded. Kinematically, the sagittal range of motion of the pelvis during the stance phase was significantly larger while walking on high heels than while walking on low heels (p < 0.05), while there was no marked difference in the lumbar angular displacement. Comparing with the walking with 0cm heel, integrated EMG of the trunk muscles were greater than those of walking on high heels of 6cm and 9cm. The results suggest that high heels result in an alternation of the pelvic range of motion in the sagittal plane. However, the influence of high heels would be absorbed by movement of the lumbar spine to maintain individual walking characteristic.